Composite

Part:BBa_M1614

Designed by: Kandy Napan   Group: UtahState_BE5930_-_S11   (2013-04-12)

Pr promoter+RBS+ XylR + terminator

Constitutive Pr promoter leads to the transcription of XYLR protein. The protein XYLR inhibits the Pr promoter when toluene is absent.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 905
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 525
    Illegal BglII site found at 1987
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 329
    Illegal NgoMIV site found at 691
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1178


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Categories
Parameters
None